Rebecca A. Prosser
Head, Undergraduate Interdisciplinary Neuroscience Concentration
Office: WLS, F-211 (865-974-2722)
Lab: WLS, A-209 (865-974-8126)
Ph.D., Univ. Illinois Urbana-Champaign, Neuroscience Program
My research focuses on circadian rhythms, largely utilizing in vitro electrophysiological recordings of the mammalian suprachiasmatic nucleus (SCN) circadian clock. Most of the current projects investigate cellular mechanisms through which mammalian circadian clock phase can be modulated, including intracellular signal transduction processes, receptor pharmacology, and extracellular signaling molecules. Recent projects have investigated the effects of neurotransmitters (e.g., serotonin, glutamate), cell adhesion molecules (e.g., PSA-NCAM) neurotrophic factors (e.g., BDNF) and extracellular proteases (e.g., tPA, MMPs). In addition to electrophysiology, our technical arsenal includes protein expression and enzyme activity assays and cell culture experiments, as well as monitoring the effects of behavioral alcohol consumption.
The research on circadian rhythms has expanded to investigate the cellular mechanisms through which alcohol modulates circadian rhythms, and the interactions between alcohol consumption and stress. This research and involves both acute in vitro administration of ethanol to brain tissue as well as self-administration of ethanol in mice. Currently the lab is investigating the influence of ethanol on protein expression in the SCN, as well as the distinct cellular underpinnings of acute vs. rapid vs. chronic ethanol tolerance. Additional research stemming from the ethanol investigations is looking into how cocaine affects the SCN clock. These projects involve collaborations with Dr. David Glass at Kent State University, and Dr. Matt Cooper at UT.
Another line of research is investigating how the tissue-type plasminogen activator (tPA) proteolytic pathway modulates BDNF signaling in the brain. This research, a collaboration with Dr. Cynthia Peterson in BCMB and Dr. Eric Mintz at Kent State University , has shown the critical nature of this pathway to activating BDNF and enhancing glutamate signaling in the SCN, and demonstrated previously undiscovered involvement of vitronectin/plasminogen activator inhibitor-1 in regulating tPA activity in the brain. Additional elements of this pathway appear to include matrix metalloproteinases (MMPs) and lipoprotein receptor-related protein-1 (LRP1).
Lindsay JH, Glass JD, Amicarelli M, and Prosser RA (2014) The mammalian circadian clock in the suprachiasmatic nucleus exhibits rapid tolerance to ethanol in vivo and in vitro. Alcohol Clin.Expt.Res. (in press)
Yamada Y, and Prosser RA (2014) Copper chelation and exogenous copper affect circadian clock phase resetting in the suprachiasmatic nucleus in vitro. Neuroscience 256: 252-261
Glass JD, Brager A, Stowie, A, and Prosser RA (2012) Cocaine modulates pathways for photic and nonphotic entrainment of the SCN circadian clock. Am.J.Physiol. Regul Integr Comp Physiol 302: R740-R750.
Mou X, Peterson CB, and Prosser RA (2009) Tissue-type plasminogen activator-plasmin-BDNF modulate glutamate-induced phase shifts of the mouse suprachiasmatic circadian clock in vitro. Eur.J.Neurosci. 30: 1451-1460.
Prosser, R.A., and Glass, J.D. (2009) The mammalian circadian clock exhibits acute tolerance to ethanol. Alcohol.Clin.Expt.Res. 33: 2088-2093.
Prosser, R.A., McElroy, B., Zakaria, A., and Glass, J.D. (2009) Ethanol modulates mammalian circadian clock phase resetting through extrasynaptic GABA receptor activation. Neuroscience 164: 842-848.