Assistant Professor, BCMB
Office: Hesler, 241: (865-974-6203)
Lab : Hesler, 216, 217: (865-974-6203)
Ph. D. Yale University
In plants intercellular communication between cells occurs via cell wall-localized, plasma membrane-lined channels called plasmodesmata. In addition to facilitating intercellular movement of water, ions and small molecules by passive diffusion, plasmodesmata also transport larger macromolecules including developmentally important transcription factors, RNA-silencing signals and viruses. The size of molecules transported by these channels is regulated during plant development. Often, this causes localized accumulation of regulatory molecules like transcription factors and microRNAs, allowing groups of cells to follow specific developmental programs.
We are interested in investigating how transport via plasmodesmata is regulated, and also in how plasmodesmata themselves are established and differentiated during plant development. Two Arabidopsis thaliana mutants with altered plasmodesmal development and function, increased size exclusion limit (ise)1 and ise2 have increased intercellular trafficking and increased numbers of plasmodesmata. Studies in these mutants reveal that plasmodesmal development and function are regulated by signals produced primarily by the chloroplasts. We are adopting two complementary approaches for understanding how these signals impinge on plasmodesmata. The first is the biochemical and molecular characterization of ISE2, which is a predicted chloroplast RNA helicase. The second approach is sophisticated morphological characterization of PD structure during development. For this we are combining state-of-the-art tissue preservation techniques coupled with transmission electron tomography and electron tomography to understand how plasmodesmal structure affects function. These studies will help illuminate the critical roles of plasmodesmata and intercellular communication during plant growth and development.
We are also investigating how pathogens take advantage of plasmodesmata for infection of their plant hosts. In this regard, we are currently investigating the coordination of the chloroplasts and plasmodesmata during viral infections and in plant immune responses.
Bobik, K. Dunlap, J.R. and Burch-Smith, T.M. (2014) Tandem high-pressure freezing and quick freeze substitution of plant tissues for transmission electron microscopy. J Vis Exp. In press.
Padmanabhan, M., Ma, S., Burch-Smith, T.M., Czymmek, K., Huijser, P. and Dinesh-Kumar, S.P. (2013) Novel positive regulatory role for the SPL6 transcription factor in the N TIR-NB-LRR receptor-mediate plant innate immunity. PLoS Pathog 9(3): e1003235
Burch-Smith, T. M., Cui, Y. and Zambryski, P. C. (2012) Reduced levels of class 1 reversibly gycosylated polypeptide increase intercellular transport via plasmodesmata. Plant Signal. Behav. 7(1): 62-7
Burch-Smith, T.M. and Zambryski, P.C (2012) Plasmodesmata paradigm shift: Regulation from without versus within. Ann Rev Plant Biol. 43 (6): 239-60
Burch-Smith, T. M., Brunkard, J. O., Choi, Y. G. and Zambryski, P. C. (2011) Organelle- nucleus cross-talk regulates plant intercellular communication via plasmodesmata. PNAS 108(51): E1451-60.
Burch-Smith, T. M. and Zambryski, P. C. (2010) Loss of INCREASED SIZE EXCLUSION LIMIT (ISE)1 or ISE2 increases the formation of secondary plasmodesmata. Current Biology 20 (11): 989-93.